Document Type : Original research article
Authors
1 Department of Plant Protection, Faculty of Agriculture, University of Zabol, Zabol, Iran
2 Institute of Agricultural Research, University of Zabol, Zabol, Iran
3 Faculty of Sciences, Department of Agriculture, University of Gonabad, Gonabad, Iran
Abstract
This study aimed to investigate the chemical compounds in the essential oil extracted from the flowering branch of Vitex agnus-castus L., grown in the Sistan region (Sistan and Baluchestan Province), and its antibacterial effects. Samples were hydrodistilled in a Clevenger–type apparatus and analyzed with gas chromatography mass spectrometry (GC-MS). In vitex essential oil, 30 bioactive compounds were identified. The most important compounds are 1,8-Cineole (39.16%), Sabinene (8.78%), β-Myrcene (6.44%), Sclareol (4.3%), and trans-Caryophyllene (3.17%). The composition of the essential oil of Vitex agnus-castus was described for the first time from Sistan region. Bacillus cereus (ATCC 11778) was the most sensitive strain against this essential oil, and Pseudomonas aeruginosa (ISIRI 275) was the most resistant strain. The monoterpenes and sesquiterpenes confer the chemical profile of the analyzed essential oil of vitex causing antibacterial effects. Further studies are required to explain the oil mechanism of action of this species involved in antimicrobial activities
Highlights
The antibacterial properties of essential oil extracted from the flowering branch of vitex grown in Sistan and Baluchestan were investigated.
Gas chromatography mass spectrometry (GC-MS) was used to analyze hydrodistilled samples.
Major compounds of vitex include 1,8-cineole, Sabinene, Myrcene, Sclareol, and trans-Caryophyllene.
Bacillus cereus was the most susceptible to this essential oil, while Pseudomonas aeruginosa was the most resistant.
More research is required to understand this species' antimicrobial oil mechanism of action.
Keywords
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