Abbas khani; Fatemeh Ordouni; Najmeh Sahebzadeh; Ali Mirshekar
Abstract
In recent years, the use of herbal compounds to control pests has been proposed as one of the alternative sources to chemical control because of their selectivity, readily biodegradable, and low impacts on non-target organisms and the environment. In this research, qualitative photochemistry and the ...
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In recent years, the use of herbal compounds to control pests has been proposed as one of the alternative sources to chemical control because of their selectivity, readily biodegradable, and low impacts on non-target organisms and the environment. In this research, qualitative photochemistry and the insecticidal effect of ethanol extract of aerial parts (without flowers) of Pimpinella stocksii Boiss. (Apiaceae) on one-day old adult aphids (Aphis gossypii) in a completely randomized design with four replications for each concentration, have been investigated under laboratory conditions (25 ± 1°C, 65 ± 5 % R. H, photoperiod 16:8 L: D). The results showed that the mortality of the tested insect increased significantly by increasing concentrations from 7 to 425 µg/cm2. In the probit analysis, an ethanol extract of Pimpinella stocksii was found to be highly toxic to Aphis gossypii Glover, with a lethal concentration of 50 percent (LC50), equal to 82.57 µg/cm2. The mortality rate of one-day adult aphids treated with an ethanol extract of P. stocksii at concentrations ranging from 7 to 425 µg/cm2 ranged from 25 to 80 percent. Preliminary phytochemical analysis showed the presence of various bioactive and insecticidal constituents in P. stocksii, like glycosides (anthraquinones), flavonoids, steroids, saponins, and triterpenoid compounds. The purpose of the study is to conduct preliminary and qualitative identification of the active components of the Pimpinella stocksii extract as well as to investigate its insecticidal activity.
Mehdi Khedri; Ali Mirshekar; Abbas Khani; Zaynab Mohkami; Hassan Ghorbani Ghouzhdi
Abstract
This study aimed to investigate the chemical compounds in the essential oil extracted from the flowering branch of Vitex agnus-castus L., grown in the Sistan region (Sistan and Baluchestan Province), and its antibacterial effects. Samples were hydrodistilled in a Clevenger–type apparatus and analyzed ...
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This study aimed to investigate the chemical compounds in the essential oil extracted from the flowering branch of Vitex agnus-castus L., grown in the Sistan region (Sistan and Baluchestan Province), and its antibacterial effects. Samples were hydrodistilled in a Clevenger–type apparatus and analyzed with gas chromatography mass spectrometry (GC-MS). In vitex essential oil, 30 bioactive compounds were identified. The most important compounds are 1,8-Cineole (39.16%), Sabinene (8.78%), β-Myrcene (6.44%), Sclareol (4.3%), and trans-Caryophyllene (3.17%). The composition of the essential oil of Vitex agnus-castus was described for the first time from Sistan region. Bacillus cereus (ATCC 11778) was the most sensitive strain against this essential oil, and Pseudomonas aeruginosa (ISIRI 275) was the most resistant strain. The monoterpenes and sesquiterpenes confer the chemical profile of the analyzed essential oil of vitex causing antibacterial effects. Further studies are required to explain the oil mechanism of action of this species involved in antimicrobial activities
